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登革熱及茲卡相關發表

資料更新日期：2024/01/03

熱帶疾病醫療暨防治中心長期研究熱帶疾病，特別是登革熱及其他蚊媒傳染病，與國內、外學者及廠商合作，包含夏威夷大學王維恭教授、新加坡國立大學Justin Chu Jang Hann副教授、國家衛生研究院陳俊宏老師、成功大學彭貴春教授等。

 

  • 登革熱臨床表現與基礎研究：與台南國立成功大學的彭貴春教授合作對急性感染期的病毒和宿主免疫反應進行了分析。藉由探討登革熱病毒感染期間造血幹細胞的活動，證明登革熱病毒感染可以誘導造血幹/前驅細胞(Hematopoietic stem and progenitor cell)活化、此活化的細胞不僅可被登革熱病毒感染，而且與病毒一同培養後可使登革熱病毒恢復感染性。這是嶄新的發現，對登革熱病毒如何入侵人類、傳播和發病機制提供了另一個探討對象。

  • 檢驗方法的驗證與比較：我們驗證多種RT-PCR 系統和快速診斷測試，以檢測台灣南部、索羅門群島發燒患者的急性樣本和蚊子中的登革病毒感染並與國家衛生研究院團隊合作檢測蚊子中的登革病毒，同時比較快篩試劑性能。

  • 血清學調查及檢測方法研發：與夏威夷大學王維恭教授合作，從研究登革病毒感染後的多克隆抗體和單克隆抗體開始，開發不同的血清學測試來區分登革病毒、茲卡病毒和其他黃病毒。這些包括以NS1為基礎的ELISA、MIA及以VLP為基礎的ELISA和西方墨點法分析。同時也比較登革病毒不同檢測原理的ELISA方法在血清陽性率研究中的性能，並調查2015年登革熱大爆發之後，高雄的登革熱病毒血清陽性率。與日本早稻田大學伊藤悅朗副教授合作，開發超高靈敏度酵素免疫分析法(Ultrasensitive ELISA with Thio-NAD Cycling)，性能媲美核酸檢測方法，並且更低成本、易於使用，適合低收入國家。

  • 登革熱疫苗及登革熱重症、治療及登革病毒基因相關研究：今年發表一篇合作論文，針對Dengvaxia登革熱疫苗在菲律賓兒童施打後，突破性感染風險分析，同時驗證上述新開發ELISA方法。研究發現接受Dengvaxia登革熱疫苗接種的登革病毒血清陰性兒童在突破性登革病毒感染後出現嚴重疾病，使得接種疫苗後的兒童得到嚴重登革熱的風險更高。開發並驗證一種能夠識別DENV-naïve Dengvaxia登革熱疫苗接受者的檢測方法至關重要。這將用於對突破性登革熱病毒感染進行更頻繁和更廣泛的評估並及時治療。


  • Tsai JJ, Chang K, Chen CH, Liao CL, Chen LJ, Tsai YY, Tsai CY, Lin PC, Hsu MC, Liu LT*. Dengue virus serotype did not contribute to clinical severity or mortality in Taiwan's largest dengue outbreak in 2015. Eur J Med Res. 2023 Nov 6;28(1):482. doi: 10.1186/s40001-023-01454-3. SCI IF 4.2. MEDICINE, RESEARCH & EXPERIMENTAL 63/136 (Q2).

  • Chen PK, Chang JH, Ke LY, Kao JK, Chen CH, Yang RC, Yoshimura T, Ito E*, Tsai JJ*. Advanced Detection Method for Dengue NS1 Protein Using Ultrasensitive ELISA with Thio-NAD Cycling. Viruses. 2023 Sep 8;15(9):1894. doi: 10.3390/v15091894. SCI IF 4.7. VIROLOGY 15/36 (Q2).

  • Dai YC, Sy AK, Jiz M, Tsai JJ, Bato J, Quinoñes MA, Reyes Mary AJ, Wang WK*. Identification of prior dengue-naïve Dengvaxia recipients with an increased risk for symptomatic dengue during fever surveillance in Philippines. Front. Immunol. Sec. Viral Immunology. Volume 14 2023 doi: 10.3389/fimmu.2023.1202055. 2023 SCI IF 7.3. IMMUNOLOGY 35/161 (Q1).

  • Tsai JJ, Tsai CY, Lin PC, Chen CH, Tsai WY, Dai YC, Lin YC, Celia Pedroso, Carlos Brites, Wang WC*. Comparing the performance of dengue virus IgG and IgG-capture enzyme-linked immunosorbent assays in seroprevalence study. BMC Infect Dis. 2023 May 8;23(1):301. doi: 10.1186/s12879-023-08307-8. SCI IF 3.7. INFECTIOUS DISEASES 51/96 (Q3).

  • Puc I, Ho TC, Chien YW, Tan SS, Fong YC, Chen YJ, Wang SH, Li YH, Chen CH, Chen PL, Perng GC, Tsai JJ *. Mobilization of hematopoietic stem and progenitor cells during dengue virus infection. Int J Mol Sci. 2022 Nov 18;23(22):14330. SCI IF 6.208. BIOCHEMISTRY & MOLECULAR BIOLOGY 69/196 (Q1).

  • Liu LT, Chen CH, Lin PC, Tsai CY, Hsu MC, Huang BY, Tsai YY, Tsai JJ *. Evaluation of a new NS1 rapid diagnostic test using a single acute‐phase serum panel collected during the largest dengue outbreak in Taiwan history in 2015. Kaohsiung J Med Sci. 2022 Apr;38(4):385-389. SCI IF 2.996. MEDICINE, RESEARCH & EXPERIMENTAL 97/139 (Q3).

  • Hsieh SC, Tsai WY, Tsai JJ, Stone M, Simmons G, Busch MP, Lanteri M, Stramer SL, Balmaseda A, Harris E, Wang WK*. Identification of Anti-Premembrane Antibody as a Serocomplex-Specific Marker To Discriminate Zika, Dengue, and West Nile Virus Infections. J Virol. 2021 Sep 9;95(19): e0061921. SCI IF 5.103. VIROLOGY 9/37 (Q1).

  • Puc I, Ho TC, Yen KL, Vats A, Tsai JJ, Chen PL, Chien YW, Lo YC*, Perng GC*. Cytokine Signature of Dengue Patients at Different Severity of the Disease. Int J Mol Sci. 2021 Mar 12;22(6):2879. SCI IF 5.924. BIOCHEMISTRY & MOLECULAR BIOLOGY 67/295 (Q1).

  • Cheng L, Liu WL, Tsou YT, Li JC, Chien CH, Su MP, Liu KL, Huang YL, Wu SC, Tsai JJ, Hsieh SL*, Chen CH*. Transgenic Expression of Human C-Type Lectin Protein CLEC18A Reduces Dengue Virus Type 2 Infectivity in Aedes aegypti.Frontiers in Immunology. 2021 Mar 9;12:640367. SCI IF 7.561. IMMUNOLOGY 24/162 (Q1).

  • Tsai WY, Driesse K, Tsai JJ, Hsieh SC, Sznajder Granat R, Jenkins O, Chang GJ, Wang WK*. Enzyme-linked immunosorbent assays using virus-like particles containing mutations of conserved residues on envelope protein can distinguish three flavivirus infections. Emerg Microbes Infect. 2020 Dec;9(1):1722-1732. SCI IF 5.776. IMMUNOLOGY 30/159 (Q1).

  • Cheng L, Liu WL, Li HH, Su MP, Wu SC, Chen HW, Pan CY, Tsai JJ*, Chen CH*. Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes. Viruses. 2020 Sep 29;12(10):1105. SCI IF 3.816. VIROLOGY 12/37 (Q2).

  • Liu LT, Chen CH, Tsai CY, Lin PC, Hsu MC, Huang BY, Wang YH, Tsai JJ*. Evaluation of rapid diagnostic tests to detect dengue virus infections in Taiwan. PLoS One. 2020 Sep 29;15(9):e0239710. SCI IF 2.74. MULTIDISCIPLINARY SCIENCES 27/71 (Q2).

  • Tyson J, Tsai WY, Tsai JJ, Mässgård L, Stramer SL, Lehrer AT, Nerurkar VR, Wang WK*. A high-throughput and multiplex microsphere immunoassay based on non-structural protein 1 can discriminate three flavivirus infections. PLoS Negl Trop Dis. 2019 Aug 23;13(8):e0007649. SCI IF 4.487. PARASITOLOGY 5/37 (Q1).

  • Tyson J, Tsai WY, Tsai JJ, Brites C, Mässgård L, Ha Youn H, Pedroso C, Drexler JF, Stramer SL, Balmaseda A, Harris E, Wang WK*. Combination of Nonstructural Protein 1-Based Enzyme-Linked Immunosorbent Assays Can Detect and Distinguish Various Dengue Virus and Zika Virus Infections. J Clin Microbiol. 2019 Jan 30;57(2):e01464-18. SCI IF 4.959. MICROBIOLOGY 24/133 (Q1).

  • Tsai JJ*, Liu WL, Lin PC, Huang BY, Tsai CY, Chou PH, Lee FC, Ping CF, Lee PA, Liu LT, Chen CH. An RT-PCR panel for rapid serotyping of dengue virus serotypes 1 to 4 in human serum and mosquito on a field-deployable PCR system. PLoS One. 2019 Mar 25;14(3):e0214328. SCI IF 2.766. MULTIDISCIPLINARY SCIENCES 24/69 (Q2).

  • Tsai JJ*, Liu WL, Lin PC, Huang BY, Tsai CY, Lee PA, Tsai YL, Chou PH, Chung S, Liu LT, Chen CH. A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos. PLoS One. 2019 Jul 10;14(7):e0218139. SCI IF 2.766. MULTIDISCIPLINARY SCIENCES 24/69 (Q1).

  • Tsai WY, Youn HH, Tyson J, Brites C, Tsai JJ, Pedroso C, Drexler JF, Balmaseda A, Harris E, Wang WK*. Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections. Emerg Infect Dis. 2018 Jul;24(7):1355-1359. SCI IF 7.422. INFECTIOUS DISEASES 4/88 (Q1)

  • Tsai JJ*, Liu LT, Lin PC, Tsai CY, Chou PH, Tsai YL, Chang HG, Lee PA. Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System. J Clin Microbiol. 2018 Apr 25;56(5):e01865-17. doi: 10.1128/JCM.01865-17. SCI IF 4.054. MICROBIOLOGY 31/127 (Q1)

  • Liu LT, Dalipanda T, Jagilly R, Wang YH, Lin PC, Tsai CY, Lai WT, Tsai JJ*. Comparison of two rapid diagnostic tests during a large dengue virus serotype 3 outbreak in the Solomon Islands in 2013. PLoS One. 2018 Aug 10;13(8):e0202304. SCI IF 2.766. MULTIDISCIPLINARY SCIENCES 15/64 (Q1).

  • Tsai JJ, Lin PC, Tsai CY, Wang YH, Liu LT*. Low frequency of asymptomatic dengue virus-infected donors in blood donor centers during the largest dengue outbreak in Taiwan. PLoS One. 2018 Oct 8;13(10): e0205248. SCI IF 2.766. MULTIDISCIPLINARY SCIENCES 15/64 (Q1).

  • Tsai JJ, Liu CK*, Tsai WY, Liu LT, Tyson J, Tsai CY, Lin PC, Wang WK*. Seroprevalence of dengue virus in two districts of Kaohsiung City after the largest dengue outbreak in Taiwan since World War II. PLoS Negl Trop Dis. 2018 Oct 24;12(10): e0006879. SCI IF 4.367. TROPICAL MEDICINE 1/20 (Q1).

  • Tsai WY, Chen HL, Tsai JJ, Dejnirattisai W, Jumnainsong A, Mongkolsapaya J, Screaton G, Crowe JE Jr, Wang WK*. Potent Neutralizing Human Monoclonal Antibodies Preferentially Target Mature Dengue Virus Particles: Implication for Novel Strategy for Dengue Vaccine. J Virol. 2018 Nov 12;92(23): e00556-18. SCI IF 4.36. VIROLOGY 8/35 (Q1).

  • Tsai WY, Youn HH, Brites C, Tsai JJ, Tyson J, Pedroso C, Drexler JF, Stone M, Simmons G, Busch MP, Lanteri M, Stramer SL, Balmaseda A, Harris E, Wang WK*. Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests. Clin Infect Dis. 2017 Nov 13;65(11):1829-1836. SCI IF 8.216. INFECTIOUS DISEASES 4/84 (Q1)

  • Tsai WY, Durbin A, Tsai JJ, Hsieh SC, Whitehead S, Wang WK*. Complexity of Neutralizing Antibodies against Multiple Dengue Virus Serotypes after Heterotypic Immunization and Secondary Infection Revealed by In-Depth Analysis of Cross-Reactive Antibodies. J Virol. 2015 Jul;89(14):7348-62. SCI IF 4.439. VIROLOGY 7/33 (Q1).

  • Hsu AY, Wu SR, Tsai JJ, Chen PL, Chen YP, Chen TY, Lo YC, Ho TC, Lee M, Chen MT, Chiu YC*, Perng GC. Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance. Sci Rep. 2015 Dec 11;5: 17990. SCI IF 5.578. MULTIDISCIPLINARY SCIENCES 5/57 (Q1).

  • Tsai JJ*, Hsieh MH, Liu LT, Hsiao HM, Perng GC. Case report mortality from septic shock in a dengue infected patient. Southeast Asian J Trop Med Public Health 2013 July;44(4):623-629. SCI IF 0.773. Tropical Medicine 16/19 (Q4).

  • Chu PY, Ke GM, Chen PC, Liu LT, Tsai YC, Tsai JJ *. Spatiotemporal dynamics and epistatic interaction sites in dengue virus type 1: a comprehensive sequence-based analysis. PLoS One. 2013 Sep.;8(9): e74165. doi:10.1371. SCI IF 3.73. MULTIDISCIPLINARY SCIENCES 7/56 (Q1).

  • Tsai JJ*, Chen PC, Liu LT, Chang K, Yao JH, Hsiao HM, Clark KB, Chen YH, Hsu Alan YH, Perng GC*. Pathogenic parameters derived from activated platelets in dengue patients. Trop Med Surg 2013 Sep;1(5): 1-7.
  • Tsai JJ, Chokephaibulkit K, Chen PC, Liu LT, Hsiao HM, Lo YC, Perng GC*. Role of cognitive parameters in dengue hemorrhagic fever and dengue shock syndrome. JOURNAL OF BIOMEDICAL SCIENCE.2013 Dec .2013; 20(1): 88. SCI IF 2.458 MEDICINE, RESEARCH & EXPERIMENTAL 57/121 (Q2).

  • Tsai JJ, Liu LT, Chang Ko, Wang SH, Hsiao HM, Clark KB, Perng GC*. The importance of hematopoietic progenitor cells in dengue. Ther Adv Hematol. 2012 Feb;3(1):59-71.

  • HE, Tsai WY, Liu IJ, Li PC, Liao MY, Tsai JJ, Wu YC, Lai CY, Lu CH, Huang JH, Chang GJ, Wu HC, Wang WK*. Analysis of epitopes on dengue virus envelope protein recognized by monoclonal antibodies and polyclonal human sera by a high throughput assay. PLoS Negl Trop Dis. 2012 Jan;6(1):e1447 (1-12). SCI IF 4.716. TROPICAL MEDICINE 1/21 (Q1).

  • Clark KB, Hsiao HM, Noisakran S, Tsai JJ, Perng GC*. Role of microparticles in dengue virus infection and its impact on medical intervention strategies. Yale J Biol Med. 2012 Mar;85(1):3-18. 

  • Tsai JJ, Jen YH, Chang JS, Hsiao HM, Norsakran S, Perng GC*. Frequency alterations in key innate immune cell components in peripheral blood of dengue patients detected by FACS analysis. J Innate Immunuty 2011;3(5):530-40. Epub 2011 Feb 18. SCI IF 4.458. IMMUNOLOGY 32/137 (Q1).

  • Chang K, Lu PL, Ko WC, Tsai JJ, Tsai WH, Chen CD, Chen YH, Chen TC, Hsieh HC, Pan CY, Harn MR*. Dengue fever scoring system: new strategy for the early detection of acute dengue virus infection in Taiwan. J Formos Med Assoc. 2009 Nov;108(11):879-85. SCI IF 1.125. MEDICINE, GENERAL & INTERNAL 76/153 (Q2).

  • Chan KS, Chang JS, Chang K, Lin CC, Huang JH, Lin WR, Chen TC, Hsieh HC, Lin SH, Lin JC, Lu PL, Chen YH, Lin CY, Tsai JJ*. Effect of serotypes on clinical manifestations of dengue fever in adults. J Microbiol Immunol Infect. 2009 Dec;42(6):471-8. SCI IF 1.631. MICROBIOLOGY 82/116 (Q3).

  • Chen TC, Lu PL, Chen YH, Tsai JJ*, Chen TP. Dengue hemorrhagic fever complicated with acute idiopathic scrotal edema and polyneuropathy. Am J Trop Med Hyg. 2008 Jan;78(1):8-10. SCI IF 2.446. TROPICAL MEDICINE 5/19 (Q2).

  • Kuo MC, Lu PL, Chang JM, Lin MY, Tsai JJ, Chen YH, Chang K, Chen HC, Hwang SJ*. Impact of renal failure on the outcome of dengue viral infection. Clin J Am Soc Nephrol. 2008 Sep;3(5):1350-6. Epub 2008 Jul 30. SCI IF 4.763. UROLOGY & NEPHROLOGY 6/69 (Q1).

  • Chen GH, Dai YC, Hsieh SC, Tsai JJ, Sy AK, Jiz M, Pedroso C, Brites C, Netto EM, Kanki PJ, Saunders DRD, Vanlandingham DL, Higgs S, Huang YS, Wang WK. Detection of anti-premembrane antibody as a specific marker of four flavivirus serocomplexes and its application to serosurveillance in endemic regions. Emerging Microbes & Infections. Accepted in 2023/12/30. SCI IF 13.2. Immunology 13/161 (Q1)